IWP-2

Small Molecules for Stem Cell Research

IWP-2 is an inhibitor of the Wnt pathway, specifically targeting membrane-bound O-acyltransferase porcupine (PORCN) with an IC50 of 27 nM. This inhibition, in turn, prevents Wnt activation, secretion, and signaling (Garcia-Reyes, et al.), which leads to decreased self-renewal of human embryonic stem cells (ESCs) (Kim, et al). IWP-2 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into functional cardiomyocytes when combined with CHIR99021, IWP-4, Activin A, bFGF, and BMP4 (Lian, et al.). Additionally, IWP-2 shows promise in the differentiation of iPSCs into corneal epithelial cells (Mikhailova, et al.).

Documents

• Technical Data Sheet
• SDS
• Brochure: Small Molecules for Stem Cell Research
• FAQs: General Guide for Small Molecules

References and Publications

• Garcia-Reyes, et al. 2018. Discovery of Inhibitor of Wnt Production 2 (IWP-2) and Related Compounds as Selective ATP-Competitive Inhibitors of Casein Kinase 1 (CK1). J Med Chem 61(9): 4087-4102.
• Mikhailova, et al. 2014. Small-molecule induction promotes corneal epithelial cell differentiation from human induced pluripotent stem cells. Stem Cell Reports. 2(2): 219-231.
• Kim, et al. 2013. Modulation of β-catenin function maintains mouse epiblast stem cell and human embryonic stem cell self-renewal. Nature Communications. 4(2403).
• Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.